Protocol Online logo
Top : Forum Archives: : Tissue and Cell Culture

tissue "fixation" - (Nov/12/2008 )


We are currently wondering how one could kill all cells in a certain tissue (e.g. skin biopsy) but retain the tissue's structure and functionality (and the cells therein) in the original state. Meaning too much crosslinking shouldn't take place. Azide and sublytic concentrations of Triton-X would lead to cell alterations (necrosis/apotosis) we don't want. I don't know about low PFA concentrations for a short incubation time.
Any ideas?



Freeze them, then cryosection?