Puromycin IC50 - (Nov/09/2008 )

Just wondering have anyone used puromycin as selection factor for HaCaT or other mammalian cells, I'm trying to find out the ic50 for HaCaT and I need like a dose range. I looked at other papers and they all have a wide range from 135uM to 0.2uM, if anyone done anything like this and could give me an approximate ic50 value that'll be awesome,

thanks in advance,

Z

hello airplane
there is this pIRES puro vector for transfection of mammalian cells. in this there is puromycin resistance gene.
they have recommended the conc as 5–10 micro-gram/ml of puromycin (#8052-1, -2), depending on
the cell line (be sure to establish a kill curve for each lot of puromycin to determine optimal effective
dose).this is for selection.
ref: http://www.clontech.com/images/pt/dis_vectors/PT3198-5.pdf
am not sure if this will help u.
good luck,
paranooid