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Chitinase - (Nov/06/2008 )

Hi there,
For chitinase assay, i need to get shrimp and crab shell powder.. anybody knows where to get it?

Thanks buddies..

Abby05

-Abby05-

Sigma sells it. You probably need to acid treat it to release the chitin from the mineral matrix. I believe it is a rather obnoxious prep with 10% sulfuric acid for a few hours at elevated temperature, followed by neutralization and filtration.

-phage434-

QUOTE (phage434 @ Nov 6 2008, 02:48 PM)
Sigma sells it. You probably need to acid treat it to release the chitin from the mineral matrix. I believe it is a rather obnoxious prep with 10% sulfuric acid for a few hours at elevated temperature, followed by neutralization and filtration.


Thanks buddy.. do appreciate it..

Abby05

-Abby05-