Chitinase - (Nov/06/2008 )
Hi there,
For chitinase assay, i need to get shrimp and crab shell powder.. anybody knows where to get it?
Thanks buddies..
Abby05
-Abby05-
Sigma sells it. You probably need to acid treat it to release the chitin from the mineral matrix. I believe it is a rather obnoxious prep with 10% sulfuric acid for a few hours at elevated temperature, followed by neutralization and filtration.
-phage434-
QUOTE (phage434 @ Nov 6 2008, 02:48 PM)
Sigma sells it. You probably need to acid treat it to release the chitin from the mineral matrix. I believe it is a rather obnoxious prep with 10% sulfuric acid for a few hours at elevated temperature, followed by neutralization and filtration.
Thanks buddy.. do appreciate it..
Abby05
-Abby05-