how to isolate the different microbiologies from a system - (Oct/28/2008 )
Hi all:
I am a rookie in this area. I want to isolate many different bacteria ( aerobic, anaerobic), fungi from a system. But I don't know how to begin and go ahead. 
So, any suggestion?
thanks!
I am a rookie in this area. I want to isolate many different bacteria ( aerobic, anaerobic), fungi from a system. But I don't know how to begin and go ahead.
So, any suggestion?thanks!
As a first stage, I would prepare a suspension of whatever system you have, and plate out onto different types of media. Choose some broad range media as well as special media, eg blood media, or media containing various environmental contaminants, heavy metals etc. Grow under both aerobic and anaerobic conditions. Also, put some of the suspension into liquid media. Grow cells at different temperatures, too, so you can pick up some of the cold-adapted bugs, assuming you're starting from a general environmental sample...
I've seen others comment previously to the same point - microbiology is not a casual excercise. Based on your comments, I don't think you know enough microbiology to be successful even if you had all the media and equipment.
I am a rookie in this area. I want to isolate many different bacteria ( aerobic, anaerobic), fungi from a system. But I don't know how to begin and go ahead.
So, any suggestion? thanks!
As a first stage, I would prepare a suspension of whatever system you have, and plate out onto different types of media. Choose some broad range media as well as special media, eg blood media, or media containing various environmental contaminants, heavy metals etc. Grow under both aerobic and anaerobic conditions. Also, put some of the suspension into liquid media. Grow cells at different temperatures, too, so you can pick up some of the cold-adapted bugs, assuming you're starting from a general environmental sample...
thanks a lot
I agree with GeorgeWolff.
You should ask your supervisor to tell you about the different techniques and culture media.
Isolating fungi and even more bacteria form the environment is not just a leisure time work; you need to be aware of the risks too! You can very easily isolate pathogenic microbes, which MUST be handeled with appropriate precautions! Otherwise you can be a danger for yourself and all the others around you!
You should ask your supervisor to tell you about the different techniques and culture media.
Isolating fungi and even more bacteria form the environment is not just a leisure time work; you need to be aware of the risks too! You can very easily isolate pathogenic microbes, which MUST be handeled with appropriate precautions! Otherwise you can be a danger for yourself and all the others around you!
I agree, ask your teacher to confirm what material kinds of microorganisms will your isolate, then choose the correspongding culture medium and method to isolate them.
Isolating microorganisms sometimes depends on your chance.Good luck!