Made an mistake during transfection! - Need to know if I corrected it by doing what I did. (Oct/22/2008 )

Hi,

I transfected my HEk293 cells with a plasmid today using lipofectamine. however during transfection i accidentally added the control vector and the vector with the gene into the same well (instead of just the control vector which it shouldve gotten). I realised this immediately and within about 10 seconds washed it twice with media and once with PBS before adding fresh media and redid the transfection.

I would like to know if the lipofectamine / dna complex wouldve acted in that short a time and if by any chance what I did to correct the situation was the right thing.

any advice would be appreciated!!

Thanks and Regards
A

I'm sure its highly unlikely that any of the DNA complex would have penetrated the cells in that time.
I'm sure you'll repeat this experiment just to make sure though ( I would)

Lost

THank you for your reply! Yeah these were controls so that ive lost so i think for preliminary results they should be okay.

i was just wondering out of curiosity... these are lipids and they go through the membrane pretty fast ( limited only by diffusion i suppose ).. and from what i remember the rates of lipids diffusing across a membrane are pretty fast ...

thx once again!