Apoptosis vurses necrosis - Way to find wether the cell death is due to apoptosis or necrosis? (Oct/21/2008 )
apobrdu i think is very reliable, otherwise acridine orange/ethidium bromide with scope is easy.
Answer for How do I determine cells are dead. 1. Change in cell morphology, 2. comparision of cell number between control and treated cell number and 3. staining method.
Here are some references for you
1. Vermes I, Haanen C, Steffens-Nakken H, Reutellingsperger C. A novel assay for apoptosis Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. Journal of Immunological Methods. 1995 Jul 17;184(1):39-51.
2. Huerta S, Goulet EJ, Huerta-Yepez S, Livingston EH. Screening and detection of apoptosis. J Surg Res. 2007 May 1;139(1):143-56.
3. Watanabe M, Hitomi M, van der Wee K, Rothenberg F, Fisher SA, Zucker R, et al. The pros and cons of apoptosis assays for use in the study of cells, tissues, and organs. Microsc Microanal. 2002 Oct ;8(5):375-91.