PVDF membrane - western blot (Sep/23/2008 )
hi everyone
I did western blot sometimes, everytime before i was ready to transfer protein to PVDF membrane, I must dip the membrane in the methanol first.
could you tell me why do i do it before transfer? what is the principle? thanks in advance!
stone
-stone757-
QUOTE (stone757 @ Sep 23 2008, 01:56 PM)
hi everyone
I did western blot sometimes, everytime before i was ready to transfer protein to PVDF membrane, I must dip the membrane in the methanol first.
could you tell me why do i do it before transfer? what is the principle? thanks in advance!
stone
I did western blot sometimes, everytime before i was ready to transfer protein to PVDF membrane, I must dip the membrane in the methanol first.
could you tell me why do i do it before transfer? what is the principle? thanks in advance!
stone
to hydrate (wet) your membrane
-Missele-
QUOTE (Missele @ Sep 23 2008, 10:23 PM)
QUOTE (stone757 @ Sep 23 2008, 01:56 PM)
hi everyone
I did western blot sometimes, everytime before i was ready to transfer protein to PVDF membrane, I must dip the membrane in the methanol first.
could you tell me why do i do it before transfer? what is the principle? thanks in advance!
stone
I did western blot sometimes, everytime before i was ready to transfer protein to PVDF membrane, I must dip the membrane in the methanol first.
could you tell me why do i do it before transfer? what is the principle? thanks in advance!
stone
to hydrate (wet) your membrane
only to hydrate the membrane? any other reasons?
-stone757-
Hi!
PVDF membranes are extremely hydrophobic and usually will not wet in aqueous solutions unless pre-wetted with methanol.
best wishes!
-Senior_Scientist-
QUOTE (Senior_Scientist @ Sep 24 2008, 01:49 AM)
Hi!
PVDF membranes are extremely hydrophobic and usually will not wet in aqueous solutions unless pre-wetted with methanol.
best wishes!
PVDF membranes are extremely hydrophobic and usually will not wet in aqueous solutions unless pre-wetted with methanol.
best wishes!
Hi
Many thanks to your reply! now I know the reseaon completely!!
Stone
-stone757-