Amyloid Beta fibril formation - (Aug/20/2008 )
Just wanted to know if anyone knows the fastest way to produce mature fibrils of A beta 1-40 of A beta 1-42
As you know A beta 1-42 forms fibrils much quicker than 1-40. The fastest way.. huh.. guess its already fast enough to form fibrils.. Its quite difficult to slow down the fibril formation to get the oligomers. I don't know the quickest way to get.. never interested to know that.
Found a paper that answers all my questions and will give me fibrils fast most people are working to slow down fibril formation to detect intermediates so papers like the one i found are hard to come by!!
Title: In vitro characterization of conditions for amyloid-beta peptide oligomerization and fibrillogenesis
Author(s): Stine WB, Dahlgren KN, Krafft GA, et al.
Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 278 Issue: 13 Pages: 11612-11622 Published: MAR 28 2003