Saccharomyces cerevisiae contamination - (Jul/29/2008 )
Hi all
I work with budding yeast and I was wondering if it is possible to have mycoplasma contamination in any of the yeast stocks or plates that I work with. If so how would I identify it?
thanks
Lisa
an interesting question
right now it is currently believed that mycoplasma species have a wider host range than previously expected, capable of moving between man and lifestock and birds.
So it might be interesting to see if the host range is actually all be a large chunk of eukaryia. And the only reason nobody has seen it before is because it is difficult see.
As for yeast playing host to mycoplasma all I have found is this
C albican and mycoplasma
Perhaps this calls for a experiment. Get some yeast, test it for mycoplasma infection.
Alternatively see if you can get it to become infected... although with over 100 species of known mycoplasma species... that might be difficult
wow----I can't believe it----thanks so much for the link-----How do you think I should check for it because I've been reading that the PCR detection isn't as fail safe as people thought
right now it is currently believed that mycoplasma species have a wider host range than previously expected, capable of moving between man and lifestock and birds.
So it might be interesting to see if the host range is actually all be a large chunk of eukaryia. And the only reason nobody has seen it before is because it is difficult see.
As for yeast playing host to mycoplasma all I have found is this
C albican and mycoplasma
Perhaps this calls for a experiment. Get some yeast, test it for mycoplasma infection.
Alternatively see if you can get it to become infected... although with over 100 species of known mycoplasma species... that might be difficult
Well, true. But it is the easiest method. There is cell staining. But I am not sure how easy these method are.
http://www.corning.com/Lifesciences/techni...ng_protocol.pdf
Come on guys - that was a casual posting years ago. Think of some of the silliness posted here.
The guy didn't say how he determined mycolasma were present but I'll bet he stained some culture material and imagined he saw something. Doubt if he ran controls or pursued it so it might have been an artifact. He certainly didn't claim infectivity.
Appears he was primary author of ~2 papers from a pathology dept in the late 90's and nothing since - importantly nothing on his discovery that he observed at the time as being so novel,
Back to the question - fungi are infected with what appear to be viruses and some yeasts carry "killer viruses" that are claimed to affect strains differentially. Maybe you'll find something but probably not. Try PCR and do run controls.
I suppose you can email but I didn't find him on the Univ Newcastle site so doubt he's there. Here's the email address he provided then:
mdkwb@mail.newcastle.edu.au.
Hello,
I had the same question before few weeks in "microbiology" forum called as "yeast contamination"... so if you want...just have a look to see the answers that I have received...