In vitro enzyme assay - (Jul/25/2008 )
Hi,
I want to ask about in vitro enzyme assay using subcellular fraction. I read in a notes i got from my lecturer. For inhibition assay we should used biochemical assay (use cell subcellular fraction) and for inhibition assay we should used cell based assay (using hepatocytes). but if i get induction result for biochemical assay, does the result valid?
TQ
-kent19-
QUOTE (kent19 @ Jul 25 2008, 07:52 AM)
Hi,
I want to ask about in vitro enzyme assay using subcellular fraction. I read in a notes i got from my lecturer. For inhibition assay we should used biochemical assay (use cell subcellular fraction) and for inhibition assay we should used cell based assay (using hepatocytes). but if i get induction result for biochemical assay, does the result valid?
TQ
I want to ask about in vitro enzyme assay using subcellular fraction. I read in a notes i got from my lecturer. For inhibition assay we should used biochemical assay (use cell subcellular fraction) and for inhibition assay we should used cell based assay (using hepatocytes). but if i get induction result for biochemical assay, does the result valid?
TQ
do both and compare; if the subcellular compartment contains the main module(s) which are affected by the drug, you have a good biochemical model to study the drug on the molecular level...
-The Bearer-