plasmid miniprep - (Jul/21/2008 )

I got a problem in agarose gel electrophoresis . I did many times the agarose of plasmid DNA of the dimethoate degrading bacteria but i could not obtain discrete band under UV, I thought it was due to improper stacking of DNA in the gel and run at low voltage for 4 hrs than usual procedure.

If you're running uncut plasmid DNA, you'll always see more than one band due to the different forms of the plasmid in your prep -- supercoiled, nicked, linear, dimers, etc.