hemocytometer - (Jul/15/2008 )

can anyone tell me that for counting of bacterial cells in hemocytometer, do we use the same trypan blue or somthing else is to be used

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It's a pain but you can use whatever stains the cells. If you have an epifluorescent set up - use DAPI. It's alot easier to count fluorescing cells.

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Check out these links too, it would be good info you will gather in this regard:
http://search.vadlo.com/b/q?keys=bacterial...mp;sn=158621799
..

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And drop-in few lines here after U find something. We want to know too And, I do not do bacteria count but just lymphocyte counts, I wanted to ask, what for do you need stain?

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thanks a lot.
link is very informative.

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Please share with us in short some of the important things here.

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We only do mammalian cells and use tryphan blue. It's not that big of a pain. I guess it all depends on what your used to!

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