Trizol extraction - (Jul/11/2008 )
TRIZOL is light sensitive and is often stored in a dark-colored, glass container covered in foil. It must be kept below room temperature. When used, it looks exactly as if it were cough syrup, bright pink. The smell, more specifically, the smell of the phenol and guanidinium thiocyanate, are extremely strong. TRIZOL works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components.
Our mission is to provide high quality end to end solutions to the BPO segment in a manner that will improve the operational efficiency while reducing the cost of the services to the client.
Sometime we don't even need DNase our RNA.
Hope this help
so you do the phenol: chloroform step to the clear phase that is removed, or to the pink/protein phase?