Problems with Bisulfite PCR - (Jul/08/2008 )
I tried a set of primers with 3 metCs, but they did not work very well. I have designed 5 primer sets so far and these are the only ones which seem to work. I ran a 2 cycle PCR along with it and it seems to have quite a bit of no-specific priming. Is this normal? Anyway to get rid of it????
Also.... My reverse primer is also methylated as the next base at the 3' is G.
you would need to optimise your annealing temperature in your PCR I would say
Thanks! I think I finally got it working!