Prostate cancer PC3 cells - Doesn't spread on glass well, doesn't migrate well, arghhh (Jul/05/2008 )
Hi all,
Was hoping that there'll be someone experienced with PC3 cells.
I'm trying to measure migration rates in stable cell lines of PC3.
In a woundhealing (scratch assay) model using video microscopy first, they don't really spread on the glass, then they didn't migrate at all (moved on the spot) for 16 hours.
In a transwell assay (0.8 um pore) which I used successfully for fibroblasts, I didn't get ANY cells migrating toward serum.
I think I need to coat the glass and the transwell filter with something. Any experience/suggestion? I have collagen or fibronectin in the lab, but can also buy in others if they are known to work.
Was hoping that there'll be someone experienced with PC3 cells.
I'm trying to measure migration rates in stable cell lines of PC3.
In a woundhealing (scratch assay) model using video microscopy first, they don't really spread on the glass, then they didn't migrate at all (moved on the spot) for 16 hours.
In a transwell assay (0.8 um pore) which I used successfully for fibroblasts, I didn't get ANY cells migrating toward serum.
I think I need to coat the glass and the transwell filter with something. Any experience/suggestion? I have collagen or fibronectin in the lab, but can also buy in others if they are known to work.
try to coat glass (poly-L-lysine, fibronectin, collagen etc)
pore size seems to be very tight; offer larger pores (3 µm or more)
Oops, I meant 8 um pores.
Thanks for the suggestions 
I was hoping someone would have tried different ECM coats so that I don't need to try all of them.
Thanks for the suggestions
I was hoping someone would have tried different ECM coats so that I don't need to try all of them.Let me make it up to you
Did you coat with Matrigel?
..