characterization of bacteriophage - (Jul/03/2008 )
Now i have bacteriophages in hand and i am going to characterize it. The characterizations that i would like to run are such as the effect of different temperature and pH on the phages activity, effect of different ions on phages and so on..so, is it i must use the fully purified phage from Cscl density gradient purification to carry out my experiment or just the PEG partial purified phages is pure enough for me to carry on the characterization?
Thanks a lot.
what would be the "impurities" that could confound your assay - and is that assay infectivity?
The impurities may present is the bacteria DNA, bacteria RNA and bacteria debris after the bacteria lysed during propagation. The assay is an infectivity assay, but i am not sure wether the "impurities" will affect my assay results.
Probably would be good to purify. i could see potential effects of variables like added cations being confounded by cellular debris.
ok, thanks a lot for your opinion.