Replication in yeast - BrdU labelling ?!? (Deoxy)Uridine incorporation?! (Jun/30/2008 )

Hello everybody,

could somebody tell me if it is possible to stain replication in yeast using BrdU labelling? Typically, it is used for detection of replication in mammalian cells. So you add BrdU directly to the culture medium where your cells are grown, and you normally processed it for immunofluorescence approach (antiBrdU antibody used as a primary antibody and fluorochrome as a secondary antibody).
But, does it work also with yeast cells? I read somewhere that wt yeast cells are not able to incorporate (deoxy)uridines into the cells. Is it true?!
Thanks for answers....

I already used successfully BrdU labelling in yeast cells using the Roche kits- no problem.

Hope it works for you as well...

Hello everybody,

could somebody tell me if it is possible to stain replication in yeast using BrdU labelling? Typically, it is used for detection of replication in mammalian cells. So you add BrdU directly to the culture medium where your cells are grown, and you normally processed it for immunofluorescence approach (antiBrdU antibody used as a primary antibody and fluorochrome as a secondary antibody).
But, does it work also with yeast cells? I read somewhere that wt yeast cells are not able to incorporate (deoxy)uridines into the cells. Is it true?!
Thanks for answers....