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cytotoxicity of FDA - (Jun/17/2008 )

Hi all,

I used fluorescein diacetate (FDA) to stain the cytoplasm of my cells a few days back, before realising i needed the cells longer. Does FDA cause cytotoxicity to cells, and does it stay in the cytoplasm or leaks out of the cell/gets metabolised? A internet search on cytotoxicity of FDA only gave results in which FDA is used to measure cytotoxicity...

Thank in advance!

-Freebird-

QUOTE (Freebird @ Jun 17 2008, 02:50 PM)
Hi all,

I used fluorescein diacetate (FDA) to stain the cytoplasm of my cells a few days back, before realising i needed the cells longer. Does FDA cause cytotoxicity to cells, and does it stay in the cytoplasm or leaks out of the cell/gets metabolised? A internet search on cytotoxicity of FDA only gave results in which FDA is used to measure cytotoxicity...

Thank in advance!


For what it's worth: my cells are still alive, are not fluorescent anymore.

-Freebird-