DAPI, permeability - (Jun/10/2008 )
QUOTE (The Bearer @ Jun 17 2008, 09:07 PM)
QUOTE (Freebird @ Jun 17 2008, 05:52 AM)
I use DAPI to stain live cells which are hard to destinguis seperately (e.g. fused myocytes), works great.
great! how to perform? DAPI in PBS? which concentration of DAPI is good? how long do you incubate with DAPI?
I use DAPI from Sigma (not sure about the stock concentration, see: http://www.sigmaaldrich.com/catalog/search...il/FLUKA/32670) working concentration is 10 ul stock per ml PBS, 30 minute incubation time.