Proliferation assay with Click-iT Invitrogen - (Jun/10/2008 )
Does anybody work with Click-iT from Invitrogen? It's a proliferation assay that uses a Flow to confirm proliferation and cell cycle.
I used EdU for proliferation and 7-AAD for cell cycle.
Can someone give me some tips?
I've seen a couple people having trouble with this kit, myself included. I'm finding that GFP expressing cells have a much higher background staining but don't seem to stain positive for incorporation. My asynchronous, non-expressing control is perfectly stained with low background in the non-Edu control. However, my GFP non-Edu control stains higher and the Edu incorporated don't stain above background (only the GFP positive cells). Needless to say, I've been very disappointed with this kit and I think they released it too quickly. I called technical support (twice) and both times they couldn't help me. Said the kit was just too new.