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Freezing mESC - (May/29/2008 )

Hi!

I was suppose to write in the other topic, but I found it didn't have much to do with that. So, for how many passages can I culture cells before freezing them for post-use? And what freezing medium would you recommend? I've tried using Bambanker at an early passage and it worked well, despite once it didn't work.

Thanks!

-Doda-

QUOTE (Doda @ May 30 2008, 01:47 AM)
Hi!

I was suppose to write in the other topic, but I found it didn't have much to do with that. So, for how many passages can I culture cells before freezing them for post-use? And what freezing medium would you recommend? I've tried using Bambanker at an early passage and it worked well, despite once it didn't work.

Thanks!


there is no really a limit for the passage number but you want to keep it as low as possible. For freesing media I use 40%FBS, 50% media, 10%DMSO.

-cmonet-

Thanks again, cmonet!
Next time I'll be trying this.

-Doda-