»ð¼±ÇóÖúmsp - M/UÌåϵ¶¼³öÏÖÌõ´ø (Sep/08/2004 )

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There are several possibilities:

First, certainly incomplete conversion of your DNA is a problem.

Both M and U pair show products may indicate there is partial methylation, or non-specific amplification by any one of the two pairs. Before you conclude what you got is partial methylation, try to optimize the conditions of your PCR reaction to rule out the possibility of non-specific amplification, such as raising the Ta. The biggest problem I think with MSP is non-specific amplification especially if PCR conditions are not optimized.

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