Photoshop help--How measure the selected area - (May/09/2008 )

If I want to use photoshop to measure the colony number and area of 2D gel image, how to do that.
I can selected all the colonies in the grey scale, then how to get the number and area?
someone can help me with that? thanks.

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I would suggest using ImageJ for this type of work. It's free and widely used, and there are a million plugins written by your fellow scientists. Here are some examples:

[attachment=4673:Cell_Colony.jpg]

In ImageJ, you can easily threshold the colonies, count them, and calculate area for each colony.

[attachment=4674:Drawing_...l_Colony.gif]

Gel Analysis

[attachment=4675:gel.gif]

Plot the peaks graphically.

[attachment=4676:Plots_of_gel_1.gif]

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Great response, brightfield!

To add a little to how to use photoshop and image J for this purpose, I would suggest the second link in the search results.

http://search.vadlo.com/b/q?sn=158621799&a...mber+&rel=0

HTH/

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