glucose transporters - (May/07/2008 )

Hi there,

so I have to write up a report like thing on glucose transporters and how "to find expression of various glucose transporter isoforms in the cortex at the mRNA level"!!!...and we have to talk about what steps to take etcetc...umm have NOOO idea where to begin other than say use trizol to extract RNA from brain??...and isolate mRNA...buuut how to tell between the diff. isoforms...any help in terms of what i should be looking up online/articles/key words would be muuuch appreciated...

cheers
b_06er

hey thx for that...soo if i was to only carry out those two techniques..would that be sufficient to write an essay on? also when using the primers would i just be using the sequence from related forms of the gene from other species?...then would i do the pcr rxn etc etc run on a gel and see what size the products are and compare to "predicted sizes" ?

cheers,
b_06er