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Hoechst AND PI for apoptosis - (May/06/2008 )

Can anyone kindly provide a protocol for Hoechst dye (membrane permeable) and PI staining for adherent cells for microscopy?

Are these two dyes enough to differentiate live cells, apoptotic cells and necrotic cells?

Thanks!

-Winston LIAO-

http://cyto.mednet.ucla.edu/Protocols/simultta.htm

-genehunter-1-