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Hi jassica,

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Nucleotide

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http://www.ensembl.org

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cacagcaaacagctccaactgcgccttcgcctcgccttccagggagcccagccaggcccactgggtattta
caagcagacctccctcgcttcagccttcctgaacccctgttagttgggaaaccacctgtctgcaccgcagcta
gagaaccgaggagaggagccgctagtctaaagggctgttggttgaaattaggaagcagtgtaaaga
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gcctggctaacatggtgaaaccccgtctctactaaaaatacaaaaaattagcgggggcatggtggcac
gcggctgtaatcccagctactcgggaggctgaggcaggagaatcgcttgaacccgggaggcggaggtt
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aaagtcttcataatttcatgggtttgcaagtatgatccaggctccccgcttctctgcaagccaatgcgagtt
aattacagcgtccgccctggtctctctccaccccacgccgtgatccattccccttctttttctccccttgtctctttc
ctcctcccccttttatttatgtatttttggttttgttttttaaggggtgttgagccgcgtctggttctagtaaacc
gaacccgctcgcgagggaggcgattggctcccgcgccggtgacggacgtggtaacgagtgcggctcgc
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CCATATTAACCAAACTAATCTCAAA (reverse)

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Hi

Do you mean you saw primer sequence in this paper "Cancer Research 64, 4717-4720, July 15, 2004" and this gene WIF-1?

the 5' flanking sequence of this gene is highly GC rich and has a long CpG island.

Where did you get the sequence? It aligns to SFRP1 gene promoter, but the URL you gave before http://www.ensembl.org/Homo_sapiens/genevi...gene_sequence=1 links to WIF-1 gene. Please make sure you have got the right one. Which gene you are trying to study?

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