Phenotypes of Knockouts - (Apr/04/2008 )
I am running RNAi experiments on crustaceans and what I am looking for is a way to guess the function of the gene deleted! Yes I know that is not really possible but what I thought might exist is a database (or similar) listing phenotypes of animals and the corresponding gene that was knocked out (or removed with RNAi, mutagenesis). Mmm, not sure if it is clear what I mean, but something like this
Type of Defect : growth
Gene affected: growth hormone producing enzyme xxx
Type of defect: neuronal
Gene affected: axo2
There are several databases for gene knockdowns in embryos. Take a look at the www.zfin.org and the www.xenbase.org sites.
At Zfin, go to the Genes/Markers/Clones page: http://zfin.org/cgi-bin/webdriver?MIval=aa-newmrkrselect.apg
Select Morpholino (the typical knockdown reagent for zebrafish) and try oep for the gene name.
thanks for the answer