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Abs for Flow - (Apr/03/2008 )

I have uncojugated cytokeratin3 mAb for immunohistochemistry and western blotting purchased from chemicon....can I use the same antibody for Flow although this application has not been mentioned in the data sheet....???? basically what is the basic difference between these two....

-Shweta Sharma-

If it works for immunohistochemistry, I would make a bet that it will work for FACS, but you should also have negative cells for control.