Cos stable transfection - (Mar/26/2008 )
Hello!
Dose anyone have experience on stable transfection on Cos cells? What should be careful in doing this? Do I have to linearize the plasmids? How much DNA and which neomicin concentration have to been used?
Thanks
Drosy
-Drosy-
Hi there,
cos7 cells are remarkable easy to transfect.
For your neomycin concentration, you will have to do a titration of your stock on your cells as this can vary greatly (unless someone else in your lab already has?? although you should really repeat everytime you get a new stock of neomycin).
good luck
-lauralee-