software for doing sequence alignments - (Mar/12/2008 )
would any of you be able to recommend a good and user-friendly software to use for sequence alignments and manipultions? thanks very much.
I use lasergene - lots of goodies
clc free workbench, geneious, vector nti. online you can find lots of different websites
I like DNAStar.
Isn't BLAST a good choice.
I too have doubt about BLAST.
the usage of blast depends on the databases you're using, if you're using the public databases you might get a lot of results - some of which might be only artifacts (anyone is allowed to upload sequences to ncbi right?)- unless it is a database created by you (with your own sequences) then the alignment could be more trustful. what is your case? are you doing alignments to existing databases or between sequences of your own?