ChIP-Chip Hybridizations - Different method for Histones vs transcription factors? (Mar/07/2008 )

Hi everyone

Just a quick question - has anyone out there done a ChIP-Chip with histone modifications? And if so, do you change any hybridisation conditions (ie: compared to TF's?). I am just wondering whether I should do my hyb's differently if I have frequent binding events (histones).
Clare

PS: Sorry - cross-posted in chromatin forum.
Have a great weekend!

Hi Clare,

as far as I know the only difference between histone mod and tf chip-chip is that you can use less starting material and less antibody for the IP with histone mod.
Apart from that everything else should be the same...

cheers

kylvalda

Hi everyone

Just a quick question - has anyone out there done a ChIP-Chip with histone modifications? And if so, do you change any hybridisation conditions (ie: compared to TF's?). I am just wondering whether I should do my hyb's differently if I have frequent binding events (histones).
Clare

PS: Sorry - cross-posted in chromatin forum.
Have a great weekend!