pink colony after electroporation of agrobacterium - (Feb/26/2008 )

Hi, all

I have transformed my construct (plasmid having chloramphenicol resistance) to agrobaterium tumefacienc (LBA4404) wiht electroporation and i got some colony on L.B plate containing Chloramphenicol as well as rifampicn. However this colony is dirty pink in colour incontrast to cramish agrobacterium colony. These colony are also grown in liquid media containing both antibiotics.

Does any body tell me that why the colour colony is change or whether it is contamination?

Preeti

hi preeti,

with electroporation u will get quite a good number of colonies...did u got all of them pinkish!!! If it is the case, the probelm may be with the medium. IF u got one or two colonies only and are in pinkish color that may be because of contamination.

thats what i think...

we never got such color with LBA/EHA/AGL/GV....strains.....

cheers
gene_tag

Serratia marcescens is a common bacterium of toilets and sinks, commonly turns up in cultures as a contaminant, but it would be unusual to find it being multi-drug resistant unless you are pouring drug containing wastes down the sink!

Thanks