HELP - 293 serum free conversion - (Jul/26/2004 )
I'm trying to convert 293 HEK cells from adherent cells growing in standard DMEM + serum to serum free media in suspension. I recently used both Gibco's offering (CD 293) and JRH's version (EX-CELL 293).
Following the protocols the cells show initial growth for a couple of days, but once I split them they proceed to stop growing and slowly die off.
Does anyone have experience with converting 293s or a favorite media/protocol?
I've been culturing 293 cells for long time, never did this happened to me. I'm using DMEM from Cellgro : 10-17-CV
Hope this helps.