yeast contamination - (Jan/22/2008 )

Hello bioforumers

Please give me a good advice for treating precious yeast contaminated cells. It's transfected Hek cells. I can't throw them away.
all the best
Missele

-Missele-

My advice though would be to toss them out., but if you really cannot and you are determined to try saving the culture you can try fungizone or Nystatin. Please see the link below:

http://www.unc.edu/depts/tcf/yeast.htm

-stemcell69-

QUOTE (stemcell69 @ Jan 24 2008, 02:46 AM)

Thank you. We will try that

-Missele-

if you treat it with harsh drugs...
will it change your cells characteristic?

what do you want to do with your cells later??

-sanjiun81-

you may try the protocol attached
successful for a colleague. He pass me the protocol about 2 years ago... and i don't see him anymore so i can just give you the protocol.

-fred_33-

QUOTE (sanjiun81 @ Jan 26 2008, 12:43 AM)

if you treat it with harsh drugs...
will it change your cells characteristic?

what do you want to do with your cells later??

Of course the answer is YES.... you will clonally select out the cells that are resistant.....AND THESE WILL NOT BE REPRESENTATIVE of a normal population of HEK cells. The answer is to prepare a master bank of these cells at the lowest possible passage number. If these are contaminated then do the transfection again.

Who said science was easy !!!!!

Rhombus

-Rhombus-

Thanks all for your good advices

-Missele-