lung cancer supernatant - what does it contain? (Dec/23/2007 )

hi!

anyone knows what does lung cancer cells supernantant contain? or any information about it? i am supporting to use the supernatant to treat glial cells. why?

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Some growth factors and lipid mediators, maybe? You ahve to be more specific on that. like what cells are these and did you treat these cells, etc.

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M working on BV2 which is a type of microlial cells. the only treatment i gave is to activate them with lipopolysaccharide (LPS).

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LPS activates so many different pathways. Cells release many cytokines like TNF-alpha, IL-2 and lipid mediators.

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different clones (of lung cancer cells) may exhibit different molecules as genehunter-1 suggests; to standardize your experiment, try to use a distinct lung cancer cell line cultured under standard conditions

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