How to determine the copy no. of different transgene - (Dec/12/2007 )
We are working on tobacco suspension culture and we transformed these cells with 3 different genes of same class (Individual transformants). To analyze these transgenes we would like to know the transgenic expression level of these genes. Is there any good method for doing that??? Thank you very much......
The question doesn't match the thread title...
To answer the title you can do Southern blots.
The other question was already answered in your post in the Biochemistry section... But you can PCR, qPCR, do Notherns.
For protein levels do western or ELISA.