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antisense oligo - (Dec/04/2007 )

Hi everyone,

Does anybody have experience with antisense oligo based cellular studies? I would appreciate any suggestions on the conditions I should first try (concentration, time of exposure, etc.) to get results. I'm trying to target my sequence with 5 of them and would really appreciate a little help.
Thanks, Jo


we looked at MCF-7 using an ASO.
we kinda nw the concentration, so didn't bother looking into that. but we did see excellent knockdown.
what i did was use a liposome to help the ASO get into the cells.
i looked at 24 and 48 hours.

if you are testing out new ASO, do the concentration, and do a time course (maybe 8, 16, 24, 48 hours).
rn a western to see changes in your favourite gene.