Does FBS in dmem inhibit the action of collagenase and elastase? - (Dec/04/2007 )
Just an update, after incubating overnight, i still can't see any cells attached at the bottom of the flask. So i threw them away. On a side note, i was also doing fibroblast primary culture using the explant method. I can see a few cells slowly moving out of the connective tissue clumps (ard 3-4mm in size) that were placed on the cell culture dish. However, it seems to be a very slow process. Is this normal?