Entamoeba histolytica - (Nov/29/2007 )

It is not really a HW question, but didn't know where else to post, so here it is.

By any chance anyone know how to grow these Entamoeba histolytica's in the lab.

This is what i could come across, just wanted to know if there is more to it.

Entamoeba histolytica required CO2 for growth in axenic culture while growth was inhibited by H2. The organism was tolerant to 5% O2 in the gas phase and it was able to detoxify products of O2 reduction in the medium. The ameba did not require a negative oxidation-reduction potential for axenic growth. However, little or no free O2 was present in media exposed to 5% O2 in the gas phase. Growth was improved by adding yeast extract to the medium.

Thank you in advance...

Perhaps not a whole lot of help, but I'd do a PubMed search of the organism and check out a few materials and methods sections. That should give you a starting point. Good luck!