Lipofectamine LTX - (Nov/20/2007 )

Hello,

Has anyone used Lipofectamine LTX from Invitrogen? The manufacturer's claim that its better than FuGENE (in terms of cytotoxicity induced and percentage of transfection). I use FuGENE and have got some very good transfections with it. I wish to know if Lipo LTX is better. I need to get a transfection reagent and Roche isn't currently in the rate contract of our instuitute whcih is why I am forced to look for other sources. Any suggestions??? Thanks in advance

Ask for a free sample and try it. (btw: Roche claims that Fugene alters expression of a lot less genes than Lipofectamine, so it's most likely commercial talk of both companies).

Maybe specifiy what cells/cell lines your working with, optimal reagents may differ between different cells.

its all advertisement. Choose any reagent that you have optimized for your cells.

The best which I had ever used. I am not attached to Invitrogen in any way but I carry 6 different cell lines and lipofectamine LTX consistently gave the highest transfection rate for all my experiments. I had tried:

1. Lipofectamine 2000k
2. Fugene
3. Verofect
4. Dreamfect Gold
5. Amaxa (electroporation)

Different cell lines give different transfection rates at different seeding conditions and exposure time (serum free/ with serum), DNA/lipid concentration

With LTX, you can also try PLUS, nupherin, combiMag, etc. Variations are endless (but your time and budget is ).

Hope this helps.