help me about testing apoptosis by facs - (Nov/16/2007 )
I am using Annexin-V and PI double staining to see if the drug could induce the cells to apoptosis, after cultured 48 hours, tested by facs. when doing compensation of fluorescence, i found the voltage of FL2 was too low, about 200, and the cell subpopulation was not significant, it was a glomerate. however, i do not know why, it is very difficult for me me to solve it. please help me.[color="#000000"][/color]
-Jack99-
QUOTE (Jack99 @ Nov 16 2007, 07:54 PM)
I am using Annexin-V and PI double staining to see if the drug could induce the cells to apoptosis, after cultured 48 hours, tested by facs. when doing compensation of fluorescence, i found the voltage of FL2 was too low, about 200, and the cell subpopulation was not significant, it was a glomerate. however, i do not know why, it is very difficult for me me to solve it. please help me.[color="#000000"][/color]
Can you show your plots? I've been using FL3 without compensation and the results were okay...
-subclavian-
maybe a stupid question, but did you use two single staining on a positive control for compensation?
-tryptofan-