i have one Q
how much is the % of CD8 or CD4 from..1 ml of blood or PBMCs

Muna,
the numbers of CD8 and CD4 will of course vary a lot between individuals, but in general:
CD8: 10-20% of PBMC
CD4 (witout CD14 which also express CD4): 20-40% of PBMC

check out separation of lymhocytes with Dynabeads from Dynal or Microbeads from Miltenyi..

Krizzy

thanx alot