Protocol Online logo
Top : Forum Archives: : Microbiology

vector pFPV25.1 - need help!!! (May/08/2004 )

I have a big problem with pFPV25.1 vector ! I haven't got its precise restriction map. i have only general map of pFPV25 vector , it is "father" of pFPV25.1 and it is without promoter .I must recut GFP (green fluorescent protein ) gene sequence from pFPV25.1(including its promoter) . I will be grateful for any information about this vector.[/COLOR]. It is for my thesis . it is very important .thanks

-tomgar-

Do you have the sequence of this vector?

-kant0008-

Have you refer to the original paper or contact the authors?

Valdivia, R. H., and S. Falkow. 1996.
Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction. Mol. Microbiol. 22:367íV378.

-Davidchan-