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methods to differentiate yeast and bacteria - (Sep/29/2007 )

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Hi,

I know it sound kind of stupid, are there any simple method to differentiate yeast and bacteria beside than microscopic?

Thanks

-madhatter-

hmmmm ....
what can be more simple than performing microscopy for yeast ????
but bacteria need to be stained for comparison

-behalbiotech-

If it is lab yeast rather than some peculiar version, then smell is the easiest. If it has white colonies and smells like baked bread, then it is yeast.

-phage434-

Actually I try to look it under microscopic. But unable to differential. Below are the pic under lactophenol blue stain and gram stain
below are two picture under microscopic using gram stain.

1st Picture is Staph Aureus.

Any idea what microorganism in the second picture. The size is more than 10x bigger than Staph Aureus. Can it be a bacteria or yeast or maybe ascospore?

-madhatter-

This is the second pic.

-madhatter-

This is another strange microorgnism uder 100x using Lactophenol BLue. The reason of this is because is won't stain on Gram Stain.

Wonder what kind of microorganism is this. Pls help

-madhatter-

I don't recognize either off-hand, but I can say that yeast cells almost always stain gram + on differential slides. in my opinion, neither is yeast

with yeast, you can also usually see budding in an actively growing culture, and depending on the strain there are often hyphae present

-aimikins-

It isn't S.pombe, S.cerevisiae or C.albicans.

-perneseblue-

QUOTE (aimikins @ Oct 5 2007, 02:29 AM)
I don't recognize either off-hand, but I can say that yeast cells almost always stain gram + on differential slides. in my opinion, neither is yeast

with yeast, you can also usually see budding in an actively growing culture, and depending on the strain there are often hyphae present



Normal yeast will have budding cell, but this one I also not sure. All this microorganism already gone thru Gamma Irradiation.
Will it be one of the cause for the yeast cell not going thru normal mitosis but stop at G-phase as a spore?

-madhatter-

all right, I'm confused

would you please tell us the source of the samples, as well as what has been done to them? it's much easier to guess their identity if we have a bit more than a pic to go on??

thanks

-aimikins-

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