Gamborg B5 preparation method - (Sep/10/2007 )
Anybody...can anybody help me?how to prepared Gamborg b5 medium?
-charger_joe-
check here:
bioprotocol
i found it through protocol online
-mdfenko-
QUOTE (mdfenko @ Sep 10 2007, 05:55 AM)
how much or gram i need to put the gamborg?
-charger_joe-
QUOTE (charger_joe @ Sep 10 2007, 01:00 PM)
QUOTE (mdfenko @ Sep 10 2007, 05:55 AM)
how much or gram i need to put the gamborg?
this is the formulation given:
Gamborg's B5 Basal Medium
0.5 g/liter MES, pH 5.7
0.05 mg/liter Kinetin
0.5 mg/liter 2,4-dichlorophenoxyacetic Acid (2,4-D)
0.8% (w/v) Agar
2% (w/v) Glucose
gamborg is the name of the person who devised this medium (it is also called: Callus Induction Medium).
-mdfenko-
QUOTE (mdfenko @ Sep 10 2007, 10:34 AM)
QUOTE (charger_joe @ Sep 10 2007, 01:00 PM)
QUOTE (mdfenko @ Sep 10 2007, 05:55 AM)
how much or gram i need to put the gamborg?
this is the formulation given:
Gamborg's B5 Basal Medium
0.5 g/liter MES, pH 5.7
0.05 mg/liter Kinetin
0.5 mg/liter 2,4-dichlorophenoxyacetic Acid (2,4-D)
0.8% (w/v) Agar
2% (w/v) Glucose
gamborg is the name of the person who devised this medium (it is also called: Callus Induction Medium).
what MES stand for?
-charger_joe-
QUOTE (charger_joe @ Sep 11 2007, 07:21 AM)
QUOTE (mdfenko @ Sep 10 2007, 10:34 AM)
QUOTE (charger_joe @ Sep 10 2007, 01:00 PM)
QUOTE (mdfenko @ Sep 10 2007, 05:55 AM)
how much or gram i need to put the gamborg?
this is the formulation given:
Gamborg's B5 Basal Medium
0.5 g/liter MES, pH 5.7
0.05 mg/liter Kinetin
0.5 mg/liter 2,4-dichlorophenoxyacetic Acid (2,4-D)
0.8% (w/v) Agar
2% (w/v) Glucose
gamborg is the name of the person who devised this medium (it is also called: Callus Induction Medium).
what MES stand for?
2-(N-Morpholino)ethanesulfonic acid
it is a pH buffer (maintains constant pH of the solution).
you're new to this stuff, aren't you?
-mdfenko-
QUOTE (mdfenko @ Sep 11 2007, 04:21 AM)
QUOTE (charger_joe @ Sep 11 2007, 07:21 AM)
QUOTE (mdfenko @ Sep 10 2007, 10:34 AM)
QUOTE (charger_joe @ Sep 10 2007, 01:00 PM)
QUOTE (mdfenko @ Sep 10 2007, 05:55 AM)
how much or gram i need to put the gamborg?
this is the formulation given:
Gamborg's B5 Basal Medium
0.5 g/liter MES, pH 5.7
0.05 mg/liter Kinetin
0.5 mg/liter 2,4-dichlorophenoxyacetic Acid (2,4-D)
0.8% (w/v) Agar
2% (w/v) Glucose
gamborg is the name of the person who devised this medium (it is also called: Callus Induction Medium).
what MES stand for?
2-(N-Morpholino)ethanesulfonic acid
it is a pH buffer (maintains constant pH of the solution).
you're new to this stuff, aren't you?
I'm very2 new to this stuff. I'm doing tissue culture project for my first degree project
-charger_joe-
QUOTE (charger_joe @ Sep 11 2007, 12:47 PM)
I'm very2 new to this stuff. I'm doing tissue culture project for my first degree project
in that case, you will require some local guidance.
in case you don't know, you will have to adjust the pH of the MES (usually with KOH) after it is dissolved otherwise it will not be at the pH you require.
good luck with your project.
-mdfenko-