# How to convert picomole to microgramme? - (Sep/07/2007 )

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Can anyone show me or tell me or explain to me..how to convert picomole to microgramme? Thanks

-cheerioet83-

I'm assuming you do mean picomole and microgram (or do you mean picomolar, and I'm assuming microgramme was a typo???), so the calculation you need is:

n=m/M
(n is number of moles, m is mass in grams and M is molecular weight in grams)

Is this a homework question??

-lauralee-

biomath calculator from promega
http://www.promega.com/biomath/default.htm

-fred_33-

QUOTE (lauralee @ Sep 7 2007, 03:37 PM)
I'm assuming you do mean picomole and microgram (or do you mean picomolar, and I'm assuming microgramme was a typo???), so the calculation you need is:

n=m/M
(n is number of moles, m is mass in grams and M is molecular weight in grams)

Is this a homework question??

Erm...this is not a homework question ..it's my weakness in calculation make me confused. Anyway thanks for the guidance.

-cheerioet83-

QUOTE (fred_33 @ Sep 7 2007, 04:55 PM)
biomath calculator from promega
http://www.promega.com/biomath/default.htm

I do find out about that...the give an assuming of one pair of nucleotide is approximately 660 pg/pmol. Does that mean is a sequence that has a length of 26bp...is 26X660 pg/pmol?

-cheerioet83-

QUOTE
I do find out about that...the give an assuming of one pair of nucleotide is approximately 660 pg/pmol. Does that mean is a sequence that has a length of 26bp...is 26X660 pg/pmol?
seems pretty obvious...

-fred_33-

QUOTE (fred_33 @ Sep 8 2007, 04:06 PM)
QUOTE
I do find out about that...the give an assuming of one pair of nucleotide is approximately 660 pg/pmol. Does that mean is a sequence that has a length of 26bp...is 26X660 pg/pmol?
seems pretty obvious...

LOL...thx for it..I already calculated it..but when i tried to compare to the labeled probe table by the company..it still have some differentiation. Can guide me in how to calculate for example:when i wanted to take out 1microgramme from a 100pmole vial with 10 micro litre, how many micro litre should i pipette out? thx

-cheerioet83-

what's the length of DNA ? RNA ? Molecular weight of molecule?...

-fred_33-

QUOTE (fred_33 @ Sep 11 2007, 08:48 PM)
what's the length of DNA ? RNA ? Molecular weight of molecule?...

The length of the DNA is 26. The molecular weight provided by the manufacturer is 8077. Besides that, the manufacturer also provide readings of microgramme which is 384.9 and pmol is 47646. Do these information help? Thanks for your kind attention and really appreciate it for your help.

-cheerioet83-

well 100pmol in 10µL makes you have 1,716µg of DNA.
as it's quite few, basically i would dilute your stock solution by 2, and take take 1,16µl of this solution

-fred_33-

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