interpretation of FACS data - (Aug/31/2007 )

Dear All
the attached file shows FACS analysis results of MiaPaCa2 human cancer cells treated with two concentrations of the same drug (1 and 10 microM) and the control vehicle treatment. apparently there is a dose-dependent G2 arrest. but the subG1 population (marked by M1 marker) looks a little bit strage to me, dose it reflect apoptotic cell death? especially I could not see floating cells at the end of drug treatment period when I examined cells under the microscope before starting FACS analysis.
thanks

Dear yobou,

Using subG1 to measure dead (apoptotic or necrotic) cells is not a valid method. Every FACS person will tell you this.

If you want to make statements about apoptosis you need to use specialized apoptosis kits.

thanks leserattepd
I am planning to do combined PI/annexin V-egfp staining to figure this out