Easiest Way to do this Seperation - Uracil from a small Peptide (Aug/30/2007 )
After a reaction, I will have a small peptide and excess free uracil in solution. I need ALL, and I mean nearly ALL The uracil gone. What is the easiest way to do this?
A way to precip the uracil w/o harming the peptide? Or rather precip the peptide?
Reacting the uracil with something?
A type of chromatography?
I am really new at this, so thanks.
1. Ultratiltration on membrane with proper MWCO.
2. SPE (solid phase extration) with C18 column/cartridge. (As good as I remember uracil is not retained by C18 HPLC columns - it should be same for SPE column/cartridge.)