seed sterlization question - how long do your seeds last? (Aug/26/2007 )

Hello,

I commonly do seed sterilization on A.thaliana seed lines. I was told that if I don't use all the sterilized seeds, that I can store them in the fridge and they will last up to 2 weeks. After that, they may not work right.

I tested this theory out with seeds that were sterilized a month ago and were stored in the fridge. I plated them on regular MS media, and they grew just fine. So now I am wondering, how long do seeds that have been sterilized typically last? Thanks.

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i don't know, but i think they become dormant in fridge conditions, and by providing them with suitable conditions, they can grown again easily....but b sure they not contaminated !

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hello,

Well first off i better say i cant answer your question, but you may be able to help me. Can you tell me where i can find info. on how to make the culture media for A.thaliana?? What is the 'regular MS media you mention??

Hope u can help

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Iam dealing with seeds of Cicer arietinum. I am new in this field I have tried to sterlize my seeds by Na hypo chlorite; SDW thrice and air dry in sterile conditions but still fungal contamination occurs. Cud you help me in telling me how to sterile the seeds.

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Hiya I am new to this area too, i have read in a few papers the method you use, -


Surface sterlisation: with a 1%(w/v) hydrochloride solution for 3 minutes and then kept in fridge at 4 degrees or a lot of papers just say the USEPA method they imerse the seeds in 10% sodium hydrochlorite fro 10 minutes.
but the method i am going to use for A.thaliana is to surface sterlise with 70% ethanol for 5 minutes, followed by soaking in 0.5%(w/v) na dodecyl sulpahte ( SDS) solution for 15 minutes and then rise with sterile water, i will use them immediately after that, and not dry them, just place them in my sterile growth gel.

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hey NK 111 if you find teh answer to that question , i would be interested to know I would liekto keep a selection of my seeds and wonder how long they will last.

Thanks
Henri

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hello

I know that I store seeds in the refrgeratory with 4 degree for more than 1 year, and they can work well.

I often store seeds after gathering and do not sterilize them. Actually, seeds are only sterilized just before germinating on the plate with 1/2 MS


stone

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hi guys,

first of all, i'm not working on arabidopsis, however, if i'm not mistaken, arabidopsis seeds need some time at 4 degrees to break dormancy and have a better germination. you shouldn't have problem at storing your seeds in a fridge, for how long you can store them also depends on how long ago you harvested them.

@ henrih: have you tried the tair? http://www.arabidopsis.org
if you're new to this i think that site could help you. good luck.

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I've germinated seeds that were sleeping for about 10 years in the fridge (Nicothiana spp).
Seeds are really though things, so, as they were conserved correctly I gave them a try.
I sterilized them before plating, because I didn't know how they were treated and obtained a fair number of plant in about 15 days.

I don't know how different it can be with Arabidopsis seeds.

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They can last for years, but gradually lose their fertility with time. If you keep them in a cool, dark place in a box containing dessicant they last best. Mutants may vary.

MS is 8 g agar and 4.24 g Murashige and Skoog salts in distilled water, adjusted to pH 5.7 and autoclaved. You can add other nutrients as well if you want - a lot of people add 3 % (v/v) sucrose.

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