Peptide (insulin) concentration device? - (Aug/10/2007 )
We want to concentrate an insulin that is commercially available from 100 units/ml to approx of 500 units/ml. But this insulin will be injected subcutaneously to humans subjects in a clinical research project. Do you have any suggestion in the way I can use to concentrate this insulin in a sterile way without introducing pyrogens or endotoxins in the insulin preparation?
Thank you very much for your help!
Have a wonderful day!
If it's in water - pool, freeze-dry and re-dissolve in smaller volume. Can't guarantee biologic activity, though...
Thank you for your input. How do you freeze-dry? Do you mean lyophilization? Do we need a special equipment do perform this?
At the begining I was thinking to use a centricon with a cut-off that will help to concentrate the sample, but in this case we would need a technique that will be safe and will not introduce pyrogens or endotoxins, and none of the concentration devices are identified to be safe for that.
You need freeze-dryer/lyophilizator of course... Freeze-dryer needs to be properly prepared to work but that's quite simple and described in user manual, then all you need is to freeze your liquid sample (at least at -30degC) and place it in / connect it to freeze-dryer.
Before you consider freeze-drying you should check if it's water solution or not, because if you dissolve dried powder in smaller volume you would increase concentration of both insulin and every other present substance eg. salts and that might be a problem for you.
Thanks for your help!
Have a great day!